coli pol I gene was used for site-directed mutagenesis (10, 11). Excellent database connection performance after flat end constructionįig. Since this clone is patented, it is not easily available for studies based on site-directed mutagenesis. The M13mp19 template carrying a 1.2-kilobase pair SacI and HindIII insert of Klenow fragment-coding sequence of E. DNA extends, fragment length increases, and Tm value increasesĢ. The enzyme synthesis activity is consistent with well-known domestic brandsįig. Synthesis of double stranded DNA in oligonucleotide directed mutagenesisĭouble deoxygenation method for DNA sequencing (Sanger method)ġ. Site-directed mutagenesis of the large fragment of DNA polymerase I (Klenow fragment) yielded two mutant proteins lacking 3,5-exonuclease activity but. Preparation of probes or markers using random primers High fidelity, strong chain switching activity, suitable for whole genome amplificationĬut off the 3 'protruding end or flatten the 5' protruding end to form a flat end Format, Enzyme supplied with 10X Reaction Buffer.
Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for sequencing Site-directed DNA mutagenesis using synthetic oligonucleotides. The enzyme exhibits DNA synthesis and proofreading (3′→5′) nuclease activities, and, in the absence of the holoenzyme’s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. Klenow Fragment (3 5 exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5 3 exonuclease activity and has mutations (D355A, E357A) which abolish the 3 5 exonuclease activity (1). Klenow Fragment is a mesophilic DNA polymerase derived from the E.
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